RNA Methylation Analysis - Global m6A Quantification from EpiGentek

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ItemRNA Methylation Analysis - Global m6A Quantification
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EpiGentek

110 Bi County Blvd. Ste 122

Farmingdale, NY 11735

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(1) The m6A regulator KIAA1429 stabilizes RAB27B mRNA and promotes the progression of chronic myeloid leukemia and resistance to targeted therapyGenes & DiseasesMarch 1, 2024Fangyi Yao, Fangmin Zhong, Junyao Jiang, Ying Cheng, Shuai Xu, Jing Liu, Jin Lin, Jing Zhang, Shuqi Li, Meiyong Li, et al.EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric) (Epigentek, USA) was utilized to measure the global m6A levels in mRNA following the manufacturer's protocol. A total of 200 ng of polyA+ mRNA was used for

(2) Transcriptome-wide N6-methyladenosine methylation profile of atherosclerosis in miceBMC GenomicsDecember 14, 2023Xinbin Zheng, Bo Zhou, Yuzhen Li, Hengren Zhong, Zhengxin Huang, Minhua Guwith over-dose anesthetic and collected the whole aorta for further studies. m6A quantification The global m6A levels were detected by EpiQuik™ m6A RNA Methylation Quantification Kit (EpiGentek, NY, USA) according

(3) Low RNA stability signifies strong expression regulatability of tumor suppressorsNucleic Acids ResearchNovember 27, 2023Xinlei Gao, Yang Yi, Jie Lv, Yanqiang Li, Kulandaisamy Arulsamy, Sahana Suresh Babu, Ivone Bruno, Lili Zhang, Qi Cao, Kaifu Chen2−ΔΔCt method with the Ct values normalized using GAPDH as an internal control. Quantification of global m6A methylation level in RNA The m6A methylation levels in PrEC and C4-2 cells were measured using EpiQuik

(4) METTL3 alters capping enzyme expression and its activity on ribosomal proteinsbioRxivNovember 26, 2023Daniel del Valle-Morales, Giulia Romano, Patricia Le, Michela Saviana, Rachel Brown, Lavender Micalo, Howard Li, Alessandro La La Ferlita, Giovanni Nigita, Patrick Nana-Sinkam, et al.motifs were generated using WebLogo (https://weblogo.berkeley.edu/) with the default settings [36]. Global m6A quantification Global m6A levels were assayed using EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric

(5) ALKBH5-mediated CHAC1 depletion promotes malignant progression and decreases cisplatin-induced oxidative stress in gastric cancerCancer Cell InternationalNovember 25, 2023Chunting Chen, Ertao Zhai, Yinan Liu, Yan Qian, Risheng Zhao, Yan Ma, Jianqiu Liu, Zhixin Huang, Jianhui Chen, Shirong Caiisolated for qPCR analysis. See Table S2 for quantitative PCR primers. m6A quantification The change of global m6A levels in mRNA was measured by EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric) (EpigenTek)

(6) Comprehensive Transcriptomic Profiling of m6A Modification in Age-Related Hearing LossBiomoleculesOctober 18, 2023Menglong Feng, Xiaoqing Zhou, Yaqin Hu, Juhong Zhang, Ting Yang, Zhiji Chen, Wei YuanmRNA primers used in our study are listed in Table A1. 2.4. m6A Quantification The alterations in global m6A levels in total mRNA were detected with an EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

(7) LncRNA FTO-IT1 promotes glycolysis and progression of hepatocellular carcinoma through modulating FTO-mediated N6-methyladenosine modification on GLUT1 and PKM2Journal of Experimental & Clinical Cancer ResearchOctober 16, 2023Fan Wang, Yuhang Hu, Hongda Wang, Ping Hu, Hewei Xiong, Zhu Zeng, Shengbo Han, Decai Wang, Jie Wang, Yong Zhao, et al.2.5 volumes of 100% ethanol at -80 °C overnight. m6A enrichment was determined by qPCR analysis. The global m6A levels in mRNA was measured with EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric) (Epigentek,

(8) METTL3 Promotes the Differentiation of Goat Skeletal Muscle Satellite Cells by Regulating MEF2C mRNA Stability in a m6A-Dependent MannerInternational Journal of Molecular SciencesSeptember 14, 2023Sen Zhao, Jiaxue Cao, Yanjin Sun, Helin Zhou, Qi Zhu, Dinghui Dai, Siyuan Zhan, Jiazhong Guo, Tao Zhong, Linjie Wang, et al.the proliferative phase (GM-3, GM-5) and differentiation phase (DM-2, DM-5) using RNAiso Plus. The global m6A methylation level of RNA was detected using the m6A RNA Methylation Quantification Kit (Epigentek, Farmingdale,

(9) ALKBH5 promotes hypopharyngeal squamous cell carcinoma apoptosis by targeting TLR2 in a YTHDF1/IGF2BP2-mediated mannerCell death discoveryAugust 23, 2023Ye J, Wu Y, Chen Y, Ren Y, Jiang X, Dong Z, Zhang J, Jin M, Chen X, Wang Z, et al.were kept in a humidified 37 °C and 5% CO2 environment, and the medium was changed every three days. Global m6A measurement Global m6A levels in mRNA were measured with an EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric

(10) In vitro study of piwi interaction RNA-31106 promoting breast carcinogenesis by regulating METTL3-mediated m6A RNA methylationTranslational Cancer ResearchJune 30, 2023Shengchao Huang, Baoying Chen, Pu Qiu, Zeming Yan, Zhongzeng Liang, Kangwei Luo, Baoyi Huang, Haiping Jiangof m6A RNA methylation Total RNA was isolated and purified from breast cancer cell lines, and the global m6A RNA methylation levels were measured using the EpiQuik m6A RNA methylation quantitative kit (EpigenTek,

(11) Comprehensive analysis of differences in N6-methyladenosine RNA methylomes in Helicobacter pylori infectionFrontiers in Cell and Developmental BiologyJune 7, 2023Huan Li, Jiahui Lin, Sha Cheng, Jingshu Chi, Ju Luo, Yu Tang, Wenfang Zhao, Yufeng Shu, Xiaoming Liu, Canxia XuEpiQuik m6A Methylation Quantification Kit (Epigentek, P-9005-96, United States) was used to measure the global m6A enrichment of mRNA. 200 ng RNA was coated in assay wells from each sample. The m6A levels are colorimetrical

(12) VIRMA promotes nasopharyngeal carcinoma, tumorigenesis, and metastasis by upregulation of E2F7 in an m6A-dependent mannerJournal of Biological ChemistryMay 1, 2023Zi-Qi Zheng, Zhuo-Hui Huang, Ye-Lin Liang, Wei-Hong Zheng, Cheng Xu, Zhi-Xuan Li, Na Liu, Pan-Yang Yang, Ying-Qin Li, Jun Ma, et al.extracted using TRIzol and subjected to mRNA separation using a GenElute mRNA Miniprep Kit (Sigma). Global m6A levels in polyadenylated RNAs were measured using an EpiQuik m6A RNA Methylation Quantification Kit

(13) m6A methyltransferase METTL3 programs CD4+ T-cell activation and effector T-cell differentiation in systemic lupus erythematosusMolecular MedicineApril 3, 2023Shuang Lu, Xingyu Wei, Huan Zhu, Zhi Hu, Meiling Zheng, Jiali Wu, Cheng Zhao, Shuang Yang, Delong Feng, Sujie Jia, et al.quantification Total RNA was isolated using TRIzol reagent according to the manufacturer’s instructions. Global m6A levels in total RNA were evaluated by the EpiQuick™ m6A RNA Methylation Quantification Kit (Colorimetric)

(14) m6A reader IGF2BP1 accelerates apoptosis of high glucose-induced vascular endothelial cells in a m6A-HMGB1 dependent mannerPeerJMarch 27, 2023Anru Liang, Jianyu Liu, Yanlin Wei, Yuan Liao, Fangxiao Wu, Jiang Ruan, Junjun LiImage-Pro Plus (IPP) 6.0 software (Media Cybernetics, Rockville, MD, USA). m6A quantification assay The global m6A levels in mRNA were measured by EpiQuik m6A RNA Methylation Quantification Kit (Colorimetrically; Epigentek,

(15) m6A eraser FTO modulates autophagy by targeting SQSTM1/P62 in the prevention of canagliflozin against renal fibrosisFrontiers in ImmunologyJanuary 4, 2023Youjing Yang, Qianmin Li, Yi Ling, Linxin Leng, Yu Ma, Lian Xue, Guoyuan Lu, Yue Ding, Jianzhong Li, Shasha TaoCorresponding secondary antibodies were added and incubated for 1 h. 2.15 m6A quantification The levels of global m6A in mRNA were measured by using an EpiQuik m6A RNA Methylation Quantification kit (Colorimetric) (EpiGentek,

(16) LncNAP1L6 activates MMP pathway by stabilizing the m6A-modified NAP1L2 to promote malignant progression in prostate cancerCancer gene therapyJanuary 1, 2023Zheng Y, Qi F, Li L, Yu B, Cheng Y, Ge M, Qin C, Li X.With the application of RNA total m6A quantification (P-9008-48-E, Epigentek, USA), we discovered that global m6A levels were decresed when METTL14 was downregulated (Fig. 2O). Further, MeRIP attested that METTL14

(17) Transcriptome-wide m6A methylation in natural yellow leaf of Catalpa fargesiiFrontiers in plant scienceJanuary 1, 2023Zhang Y, Wang J, Ma W, Lu N, Fu P, Yang Y, Zhao L, Hu J, Qu G, Wang N.selection were performed to measure the global change of the m6A modification level. The change of global m6A levels in mRNA was measured by EpiQuik m6A RNA Methylation Quantification Kit (EpiGentek, P-9005, NY,

(18) LRPPRC facilitates tumor progression and immune evasion through upregulation of m(6)A modification of PD-L1 mRNA in hepatocellular carcinomaFrontiers in immunologyJanuary 1, 2023Wang H, Tang A, Cui Y, Gong H, Li H.for eluting RNA. RT-qPCR was implemented on the immunoprecipitated RNA for detect the enrichment. Global m6A quantification Global m6A level was measured utilizing EpiQuik™ m6A RNA Methylation Quantification

(19) ALKBH5 promotes PD-L1-mediated immune escape through m6A modification of ZDHHC3 in gliomaCell Death DiscoveryDecember 24, 2022Wenhui Tang, Ningbo Xu, Jian Zhou, Zhenyan He, Cameron Lenahan, Chenyang Wang, Huangyi Ji, Boyang Liu, Yujiao Zou, Huijun Zeng, et al.Fluorescence-minus-one, unstained, and single-stained cells were also used to set gates. m6A quantification The global m6A levels in total RNAs were measured using the EpiQuik m6A RNA Methylation Quantification Kit (Epigentek,

(20) A programmable system to methylate and demethylate N6-methyladenosine (m6A) on specific RNA transcripts in mammalian cellsJournal of Biological ChemistryNovember 1, 2022Chen Chang, Gang Ma, Edwin Cheung, Andrew P. Hutchinssubstrate (Bio-Rad) according to the manufacturer's instructions. Whole transcriptome m6A measurements Global m6A/m in total RNA was quantified by the EpiQuik m6A/m RNA Methylation Quantification Kit (Epigentek Group)

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RNA Methylation Analysis - Global m6A Quantification from EpiGentek

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RNA Methylation Analysis - Global m6A Quantification from EpiGentek

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